电子邮箱: yaols@qibebt.ac.cn

电话：0532-80662792 

个人经历：

     2010-06至今, 中科院青岛生物能源与过程研究所,研究员；

    2006-06至2010-05,美国国立健康中心, 博士后；

    2001-07至2006-05 密西根州立大学，物理化学博士。

承担项目：

 主持中组部WR人才计划项目

 山东省“泰山学者特聘专家”项目（优秀）

 国家重点研发计划子课题2项

 国家自然科学基金面上项目4项

 国家自然科学基金国际合作项目1项

 中科院BR人才计划项目

 山东省“杰出青年”基金等项目

研究方向：

1）催化抗体药物设计与研发；

2）蛋白质作用机制解析；

3）酶的理性设计

代表性论文：

(1) Xiangfei Song#,; Mengting Wang#;Xiaoxu Chen,; Xueying Zhang,; Ying Yang,; Zhijun Liu,; Wang; Lishan Yao*,.  Quantifying Protein Electrostatic Interactions in Cells by Nuclear Magnetic Resonance Spectroscopy.J. Am. Chem. Soc.,2021, doi: org/10.1021/jacs.1c10154.

(2) Mengting Wang#，;Xiangfei Song#,;jingfei Chen, Xiaoxu Chen,Xueying Zhang,Ying Yang,Zhijun Liu,and Lishan Yao*, Intracellular Environment Can Change Protein Conformational Dynamics in Cells through Weak Interactions.Science Advances 2023, http://www.science.org/doi/10.1126/sciadv.adg9141

(3) Song Xiangfei; LvTianhang; Chen Jingfei; Wang Jia; Yao Lishan*. Characterization of Residue Specific Protein Folding and Unfolding Dynamics in Cells. Journal of the American Chemical Society, 2019, 141 (29), 11363-11366.

(4) Li Jingwen#; Wang Yefei#; An Liaoyuan; Chen Jingfei;Yao Lishan*. Direct Observation of CH/CH van der Waals Interactions in Proteins by NMR. Journal of the American Chemical Society, 2018, 140, 3194-3197.

(5) Zhang Ning#; An Liaoyuan#; Li Jingwen; Liu Zhijun; Yao Lishan*. Quinary Interactions Weaken the Electric Field Generated by Protein Side-Chain Charges in the Cell-like Environment. Journal of the American Chemical Society, 2017, 139, 647-654.

(6) Li Jingwen#; Wang Yefei#; Chen Jingfei; Liu Zhijun; Bax Ad; Yao Lishan*. Observation of alpha-Helical Hydrogen-Bond Cooperativity in an Intact Protein. Journal of the American Chemical Society, 2016, 138, 1824-1827.

(7) An Liaoyuan; Wang Yefei; Zhang Ning; Yan Shihai; Bax Ad; Yao Lishan*. Protein Apparent Dielectric Constant and Its Temperature Dependence from Remote Chemical Shift Effects.Journal of the American Chemical Society, 2014, 136, 12816−12819.

(8) WangYefei#; An Liaoyuan#; Yang Ying; Yao Lishan*.Generating FiveIndependent Molecular Alignments for Simultaneous Protein Structure andDynamics Determination Using Nuclear Magnetic Resonance Spectroscopy. Analytical Chemistry, 2020,92(23):15263-15269.

(9) Xiaoxu Chen,# Xueying Zhang,# Mingming Qin,# Jingfei Chen, Mengting   Wang, Zhijun Liu, Liaoyuan An, Xiangfei   Song,* and Lishan Yao*, Protein Allostery Study in Cells Using NMR   Spectroscopy, Anal. Chem. 2024, 96,   7065−7072  

(10) Zhang Ning#; Wang Yefei#; An Liaoyuan; Song Xiangfei; HuangQingshan; Liu Zhijun; Yao Lishan*. Entropy Drives the Formation of Salt Bridges in the Protein GB3.AngewandteChemie International Edition, 2017, 56, 7601-7604.

授权发明专利：

1. 姚礼山，宋乡飞，王业飞，一种改良性能的黄素单核苷酸结合荧光蛋白质，2012年，中国，ZL 201210160241.3.

2. 姚礼山，宋乡飞，王业飞，张树军，张雨，洪景波.一种改良性能的纤维素内切酶Cel7B突变体. 2015年，中国, ZL 201510049724.X

3. 姚礼山; 邓涵中; 王业飞; 秦鸣铭; 宋乡飞 ; 一种酵母胞嘧啶脱氨酶融合蛋白及其突变体和应用,2023年, 中国, ZL 202310073315.8 (专利)

4. 姚礼山; 梅硕·哈杜什·贝赫; 宋乡飞; 王梦婷 ; 具有高酶活性与高热稳定性的葡聚糖单加氧酶及其制备方法与应用, 2023年, 中国, ZL 202310083991.3 (专利)