姓 名：姚礼山  研究组组长

职 称：研究员

研究领域：蛋白质核磁共振、蛋白质理性设计

邮箱：yaols@qibebt.ac.cn

个人简历：

教育经历：

2010-06至今,中科院青岛生物能源与过程研究所,研究员；

2006-06至2010-05,美国国立健康中心,博士后；

2001-07至2006-05密西根州立大学，物理化学博士。

个人荣誉：

荣获中组部WRJH、中科院和山东省TSTP人才项目称号

研究方向：

1）人工酶和抗体酶药物设计与研发；

2）蛋白质结构和动态性研究的新技术和新方法发展；

代表性论文：

(1)Xiangfei Song^#,; Mengting Wang^#;Xiaoxu Chen,; Xueying Zhang,; Ying Yang,; Zhijun Liu,; Wang; Lishan Yao*,.  Quantifying Protein Electrostatic Interactions in Cells by Nuclear Magnetic Resonance Spectroscopy.J. Am. Chem. Soc.,2021, doi: org/10.1021/jacs.1c10154.

(2)Mengting Wang^#，;Xiangfei Song#,;jingfei Chen, Xiaoxu Chen,Xueying Zhang,Ying Yang,Zhijun Liu,and Lishan Yao*, Intracellular Environment Can Change Protein Conformational Dynamics in Cells through Weak Interactions.Science Advances 2023, http://www.science.org/doi/10.1126/sciadv.adg9141

(3) Song Xiangfei; LvTianhang; Chen Jingfei; Wang Jia; Yao Lishan*. Characterization of Residue Specific Protein Folding and Unfolding Dynamics in Cells. Journal of the American Chemical Society, 2019, 141 (29), 11363-11366.

(4) Li Jingwen^#; Wang Yefei^#; An Liaoyuan; Chen Jingfei;Yao Lishan*. Direct Observation of CH/CH van der Waals Interactions in Proteins by NMR. Journal of the American Chemical Society, 2018, 140, 3194-3197.

(5) Zhang Ning^#; An Liaoyuan^#; Li Jingwen; Liu Zhijun; Yao Lishan*. Quinary Interactions Weaken the Electric Field Generated by Protein Side-Chain Charges in the Cell-like Environment. Journal of the American Chemical Society, 2017, 139, 647-654.

(6) Li Jingwen^#; Wang Yefei^#; Chen Jingfei; Liu Zhijun; Bax Ad; Yao Lishan*. Observation of alpha-Helical Hydrogen-Bond Cooperativity in an Intact Protein. Journal of the American Chemical Society, 2016, 138, 1824-1827.

(7)An Liaoyuan; Wang Yefei; Zhang Ning; Yan Shihai; Bax Ad; Yao Lishan*. Protein Apparent Dielectric Constant and Its Temperature Dependence from Remote Chemical Shift Effects.Journal of the American Chemical Society, 2014, 136, 12816−12819.

(8)WangYefei^#; An Liaoyuan^#; Yang Ying; Yao Lishan*.Generating FiveIndependent Molecular Alignments for Simultaneous Protein Structure andDynamics Determination Using Nuclear Magnetic Resonance Spectroscopy. Analytical Chemistry, 2020,92(23):15263-15269.

(9) Li Jingwen^#; Chen Jingfei^#;An Liaoyuan; Yao Lishan*. Polyol and sugar osmolytes can shorten protein hydrogen bonds to modulate function. Communications Biology, 2020, 3(1): 528.

(10) Zhang Ning^#; Wang Yefei^#; An Liaoyuan; Song Xiangfei; HuangQingshan; Liu Zhijun; Yao Lishan*. Entropy Drives the Formation of Salt Bridges in the Protein GB3.AngewandteChemie International Edition, 2017, 56, 7601-7604.

授权发明专利：

1.姚礼山，宋乡飞，王业飞，一种改良性能的黄素单核苷酸结合荧光蛋白质，2012年，中国，ZL 201210160241.3.

2.姚礼山，宋乡飞，王业飞，张树军，张雨，洪景波.一种改良性能的纤维素内切酶Cel7B突变体. 2015年，中国, ZL 201510049724.X